Flow Cytometry Core
Flow Cytometers are used to determine the characteristics of the cells in a complex cell mixture and then cell populations of interest can be collected on the cell sorter for further experimentation. The Flow Cytometry core provides researchers with high-speed cell sorting services and access and training on flow analyzers, as well as software training, consultation services, and regular instrument maintenance. The facility is overseen by a faculty Scientific Advisor, Dr. Kevin Urdahl.
Flow Cytometry Equipment
- BD LSRII analyzers: We have two LSRIIs that are equipped with four lasers (violet, blue, green and red) and capable of detecting up to 18 colors. Both include a high throughput sampler that can rapidly introduce samples directly from 96 and 384 well plates.
- BD FACS AriaII cell sorter: Equipped with four lasers (violet, blue, green and red) and capable of detecting up to 18 colors simultaneously. This sorter is located in a Biosafety Level 3 (BSL-3) facility and is housed in a biosafety hood to allow for sorting BSL-3 level infectious agents.
- Cytopeia Influx cell sorter: Equipped with three lasers (ultraviolet, blue and red) and capable of detecting eight colors. The sorter also has multiple nozzle sizes for sorting larger cells such as hepatocytes.
For information on pricing and scheduling, or to discuss your upcoming project, contact the Flow Cytometry Core Manager, Weldon DeBusk via email or call (206) 256-7164.