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Global Health Biotechnology Center: Imaging
This core provides the tools for capturing and analyzing
images, whether they come from cells viewed through a microscope or from
molecules identified on gels and blots. The fluorescence microscope and
quantitative autoradiography imaging equipment and software allows researchers
to describe cell changes and function under varying conditions and to
characterize molecules following separation on gels.
The facility is equipped with a DeltaVision RT
optical/digital-sectioning microscope with deconvolution software for 3D
live-cell imaging in real time, as well as two fluorescence microscopes: a Nikon
Eclipse E600 and Nikon Eclipse TE2000U inverted microscope. The microscopes are
equipped with high-speed Cool Snap CCD cameras, phase contrast capabilities and
fluorescence filters. This facility also has a Storm 860 phosphorimager capable
of scanning storage phosphor plates for the detection of radioactive samples and
for scanning a variety of fluorescent and chemiluminescent signals from gels and
blots.
The facility has the following equipment:
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Applied Precision DeltaVision RT
optical/digital-sectioning microscope – For optimal 3D live-cell
imaging in real time. This specialized instrument is equipped with
DIC optics for visible imaging and up to 6 channels (DAPI, FITC,
Texas Red, CFP, YFP, and Cy-5) for capturing fluorescently labeled
cells. The SoftWorx software package comes with deconvolution
capabilities for the highest quality images.
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Nikon Eclipse E600 upright microscope -
This scope has 3 filters for fluorescence imaging (FITC, DAPI, and
Texas Red) and is equipped with both a high speed Cool Snap CCD
camera and a true color camera. The true color camera is used for
chromatic staining in tissue samples. Metamorph analysis software is
available.
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Nikon TE2000U inverted microscope - This
scope has phase contrast and DIC optics for visible imaging, 3
filters for fluorescence imaging (FITC, DAPI, and Texas Red), a
motorized stage, and a high speed Cool Snap CCD camera. There are
multiple options for viewing slides, Petri dishes, chamber slides,
multi-well plates and tissue culture flasks. Metamorph analysis
software is available.
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Odyssey Imaging System - Applications
include one- and two-color Western blots, in-gel fluorescence
detection (acrylamide and agarose gels), quantitative fluorescence
detection in standard microtiter plates (in-cell western),
immunohistochemistry of tissue sections, and general imaging of
coomassie stained gels and membranes. Check the
LI-COR web site for further applications and recent
publications.
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Storm 860 phosphorimager - This instrument
is capable of scanning a variety of fluorescent and chemiluminescent
signals from gels, blots and phosphor screens.
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Imaging Core External User Fees |
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Fluorescence microscopes |
DeltaVision RT |
Phosphorimager |
Odyssey |
| Contact
Core Manager for pricing
(206) 256-7498. |
To get information about SBRI's Imaging facility, please contact the
Core Manager, Tracy Mitchell, (206)
256.7498.
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