Global Health Biotechnology Center: Flow Cytometry

This core provides high-speed cell sorting, flow cytometer analysis, and related consultation services by qualified scientific and technical staff to determine the characteristics of the cells in a complex cell mixture. Cell populations of interest can then be collected on the cell sorter for further experimentation. This facility also includes surface plasmon resonance instrumentation for real-time monitoring of macromolecular interactions.

The facility is equipped with the following:

• Cytopeia Influx high-speed cell sorter – Equipped with 3 lasers (UV, blue, red) for experiments with up to 8 colors; includes a biocontainment system for sorting BSL-2 level infectious agents.

• Becton Dickinson LSRII analyzer – Equipped with 4 lasers (violet, blue, green, red) for experiments up to 18 colors; includes a high throughput sampler (HTS) that can rapidly introduce samples from 96 and 384 well plates.

• Beckman Coulter Epics XL-MCL cell analyzer – Equipped with a blue laser for experiments with up to 4 colors; includes automated multi-tube carousel loader (100 tubes/hour).

• Biacore X - Optical biosensor instrument for affinity measurements, binding kinetics and binding specificity experiments.

This core is overseen by a faculty Scientific Advisor, Dr. Helen Horton, and is operated by a Core Manager and technical staff.

To get more information about SBRI's Flow Cytometry facility, please contact the Core Manager, Sean Gray, (206) 256.7143.

HOME | CONTACT US | SITEMAP | DONATE | POLICY

©2008 Seattle Biomedical Research Institute. All rights reserved.

SBRI is a 501(c)3 not-for-profit organization. info@sbri.org