Template Quality
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The quality of sequence is dependent on the quality of the template
DNA and the respective primers. DNA and primers should be free of
proteins, salts, and organics. PCR template should be free of
extra primers and dNTPs. For the highest quality plasmid DNA, we
recommend using
Eppendorf Fast Plasmid Kit or
Millipore Montage
96-well plasmid prep.
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